PAR2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PAR2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 12160-1-AP

Synonyms

F2RL1, G protein coupled receptor 11, GPR11, PAR 2, PAR2, Thrombin receptor like 1

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Validation Data Gallery

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  WB analysis of COLO 320 using 12160-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12160-1-AP (PAR2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: COLO 320 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Product Information

12160-1-AP targets PAR2 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAR2 fusion protein Ag2801
• Full Name: coagulation factor II (thrombin) receptor-like 1
• Calculated Molecular Weight: 397 aa, 44 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC018130
• Gene Symbol: PAR2
• Gene ID (NCBI): 2150
• RRID: AB_10598009
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteinase-activated receptors (PARs) are G protein-coupled receptors activated through cleavage of their N-termini by mainly serine proteases. The family of PARs includes four members: PAR1, PAR2, PAR3, and PAR4. PAR2, also known as F2RL1, is expressed in epithelial cells (e.g., lung, gastrointestinal tract), endothelial cells, smooth muscle cells, fibroblasts, nerves, and some immune and inflammatory cells. PAR2 knockout mice and PAR2 agonists and antagonists have implicated PAR2 as a promising target in inflammatory conditions; respiratory, gastrointestinal, metabolic, cardiovascular, and neurological dysfunction; and cancers. (PMID:23895492)

Publications

Species	Application	Title
		J Med Chem; Toward drugs for protease-activated receptor 2 (PAR2).; Authors - Mei-Kwan Yau
human	WB	Cells; Modulation of Intestinal Epithelial Permeability via Protease-Activated Receptor-2-Induced Autophagy.; Authors - Yuju Kim
human	WB	Biochem J; Protease-Activated Receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway.; Authors - Chunyang Du
human	WB	Oral Dis; Linagliptin's impact on lymphatic barrier and lymphangiogenesis in oral cancer with high glucose; Authors - Hongyu Wang
human	WB	Med Oncol; Long non-coding RNA AC245100.4 contributes to prostate cancer migration via regulating PAR2 and activating p38-MAPK pathway.; Authors - Chi Liu ; KD Validated
human	WB	Heliyon; Long non-coding RNA AC245100.4 activates the PI3K/AKT pathway to promote PCa cell proliferation by elevating PAR2; Authors - Ke Zhang