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  • KD/KO Validated

PAR2 Polyclonal antibody

PAR2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 12160-1-AP

Synonyms

F2RL1, G protein coupled receptor 11, GPR11, PAR 2, PAR2, Thrombin receptor like 1



Tested Applications

Positive WB detected inCOLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12160-1-AP targets PAR2 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAR2 fusion protein Ag2801
Full Name coagulation factor II (thrombin) receptor-like 1
Calculated Molecular Weight 397 aa, 44 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC018130
Gene Symbol PAR2
Gene ID (NCBI) 2150
RRIDAB_10598009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proteinase-activated receptors (PARs) are G protein-coupled receptors activated through cleavage of their N-termini by mainly serine proteases. The family of PARs includes four members: PAR1, PAR2, PAR3, and PAR4. PAR2, also known as F2RL1, is expressed in epithelial cells (e.g., lung, gastrointestinal tract), endothelial cells, smooth muscle cells, fibroblasts, nerves, and some immune and inflammatory cells. PAR2 knockout mice and PAR2 agonists and antagonists have implicated PAR2 as a promising target in inflammatory conditions; respiratory, gastrointestinal, metabolic, cardiovascular, and neurological dysfunction; and cancers. (PMID:23895492)

Protocols

Product Specific Protocols
WB protocol for PAR2 antibody 12160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Med Chem

Toward drugs for protease-activated receptor 2 (PAR2).

Authors - Mei-Kwan Yau
humanWB

Cells

Modulation of Intestinal Epithelial Permeability via Protease-Activated Receptor-2-Induced Autophagy.

Authors - Yuju Kim
humanWB

Biochem J

Protease-Activated Receptor-2 promotes kidney tubular epithelial inflammation by inhibiting autophagy via the PI3K/Akt/mTOR signalling pathway.

Authors - Chunyang Du
humanWB

Oral Dis

Linagliptin's impact on lymphatic barrier and lymphangiogenesis in oral cancer with high glucose

Authors - Hongyu Wang
humanWB

Med Oncol

Long non-coding RNA AC245100.4 contributes to prostate cancer migration via regulating PAR2 and activating p38-MAPK pathway.

Authors - Chi Liu
  • KD Validated
humanWB

Heliyon

Long non-coding RNA AC245100.4 activates the PI3K/AKT pathway to promote PCa cell proliferation by elevating PAR2

Authors - Ke Zhang