PAR1/Thrombin Receptor Polyclonal antibody

• Featured Product
• KD/KO Validated

PAR1/Thrombin Receptor Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 26366-1-AP

Synonyms

F2R, CF2R, Proteinase-activated receptor 1, PAR-1, PAR1

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Validation Data Gallery

• 

  WB analysis using 26366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26366-1-AP (PAR1/Thrombin Receptor antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26366-1-AP

  WB result of F2R antibody (26366-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAR1/Thrombin Receptor transfected K-562 cells.

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  IHC staining of human liver using 26366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 26366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Y79 cells, K-562 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

26366-1-AP targets PAR1/Thrombin Receptor in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAR1/Thrombin Receptor fusion protein Ag24378
• Full Name: coagulation factor II (thrombin) receptor
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC051909
• Gene Symbol: F2R
• Gene ID (NCBI): 2149
• RRID: AB_2880492
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protease-activated receptor-1 (PAR1) is a G protein coupled receptor.PAR1 has a greater affinity for thrombin and mediates rapid Ca2+ mobilization and platelet activation. PAR1 has a calculated molecular weight of 47 kDa, but can be detected as 70 kDa after posttranslational modification.(PMID: 26822533)

Publications

Species	Application	Title
human,mouse	WB,IHC,IP	Breast Cancer Res Treat; Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT.; Authors - Yihui Zhu
hamster	WB	Leukemia; Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15; Authors - Cong Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (02-15-2024)

Several unspecific bands detected by WB one of which was the correct size. IF staining worked well but caveated by potential lack of specificity.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1 in 1000 WB 1 in 200 IF
• Cell Tissue Type: HUVEC lysate