F2 Monoclonal antibody

F2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E12A8

Cat no : 66509-1-Ig

Synonyms

Coagulation factor II, Prothrombin, EC:3.4.21.5, Activation peptide fragment 2, Activation peptide fragment 1

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Validation Data Gallery

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  WB analysis of human plasma using 66509-1-Ig

  Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66509-1-Ig (F2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of human plasma using 66509-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66509-1-Ig (F2 antibody) at various dilutions incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 66509-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66509-1-Ig (F2 antibody) at dilution of 1:150 (under 10x lens).

• 

  IHC staining of human liver using 66509-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66509-1-Ig (F2 antibody) at dilution of 1:150 (under 40x lens).

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Tested Applications

• Positive WB detected in: human plasma tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66509-1-Ig targets F2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: F2 fusion protein Ag18385
• Full Name: coagulation factor II (prothrombin)
• Calculated Molecular Weight: 622 aa, 70 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC051332
• Gene Symbol: F2
• Gene ID (NCBI): 2147
• RRID: AB_2881872
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II, also known as prothrombin or factor II, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.