Factor XIIIa Polyclonal antibody

Factor XIIIa Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17223-1-AP

Synonyms

F13A1, Transglutaminase A chain, Protein-glutamine gamma-glutamyltransferase A chain, F13A, EC:2.3.2.13

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Validation Data Gallery

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  WB analysis of mouse lung using 17223-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17223-1-AP (Factor XIIIa antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human normal colon using 17223-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17223-1-AP (Factor XIIIa antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human normal colon; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17223-1-AP targets Factor XIIIa in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Factor XIIIa fusion protein Ag11059
• Full Name: coagulation factor XIII, A1 polypeptide
• Calculated Molecular Weight: 732 aa, 83 kDa
• Observed Molecular Weight: 80-85 kDa
• GenBank Accession Number: BC027963
• Gene Symbol: Factor XIIIa
• Gene ID (NCBI): 2162
• RRID: AB_2878363
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Factor XIIIa is a blood proenzyme that catalyzes intermolecular cross-linking of fibrinogen and is involved in clotting cascade. It has been identified in various tissues including placenta, uterus, and prostate. Anti- Factor XIIIa has been found to be useful in differentiating between dermatofibroma (90% positive) and desmoplastic malignant melanoma (negative). Factor XIIIa positivity is also seen in capillary hemagioblastoma, hemangioendothelioma, hemangiopericytoma, xanthogranuloma, xanthoma, hepatocellular carcinoma, glomus tumor, and meningioma.