Factor XII Polyclonal antibody

Factor XII Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27154-1-AP

Synonyms

Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor

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Validation Data Gallery

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  WB analysis of human plasma using 27154-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 27154-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 27154-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 27154-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27154-1-AP (F12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 27154-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27154-1-AP (F12 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 27154-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27154-1-AP (F12 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: human plasma tissue, K-562 cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27154-1-AP is Factor XII Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Factor XII fusion protein Ag25912
• Full Name: coagulation factor XII (Hageman factor)
• Calculated Molecular Weight: 615 aa, 68 kDa
• Observed Molecular Weight: 80 kDa and 52 kDa
• GenBank Accession Number: BC012390
• Gene Symbol: Factor XII
• Gene ID (NCBI): 2161
• RRID: AB_2880778
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Factor XII (F XII, Hageman factor) is a 80kD, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.