Ezrin Polyclonal antibody

• Featured Product
• KD/KO Validated

Ezrin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA

Conjugate

Unconjugated

Publications

15

Cat no : 26056-1-AP

Synonyms

Villin-2, VIL2, EZR

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Validation Data Gallery

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  WB analysis using 26056-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 26056-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 26056-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 26056-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26056-1-AP (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 26056-1-AP

  IP result of anti-Ezrin (IP:26056-1-AP, 4ug; Detection:26056-1-AP 1:2000) with HeLa cells lysate 3200ug.

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  IHC staining of mouse stomach using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:8000 (under 40x lens).

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  IHC staining of human small intestine using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 10x lens).

• 

  IHC staining of human small intestine using 26056-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 26056-1-AP (Ezrin antibody) at dilution of 1:2000 (under 40x lens).

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  IF Staining of HeLa using 26056-1-AP

  Immunofluorescent analysis of HeLa cells using 26056-1-AP (Ezrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 26056-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Ezrin Polyclonal antibody (26056-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse kidney tissue, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse stomach tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

26056-1-AP targets Ezrin in WB, IHC, IF/ICC, FC (Intra), IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Ezrin fusion protein Ag23530
• Full Name: ezrin
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC013903
• Gene Symbol: Ezrin
• Gene ID (NCBI): 7430
• RRID: AB_2722561
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	IF	Acta Neuropathol; Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.; Authors - Matthew J Broadhead
human	WB	Proc Natl Acad Sci U S A; Cotranslational interaction of human EBP50 and ezrin overcomes masked binding site during complex assembly.; Authors - Krishnendu Khan ; KO Validated
mouse	WB	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
human	WB	Int J Mol Sci; Bidirectional Tumor-Promoting Activities of Macrophage Ezrin.; Authors - Krishnendu Khan ; KD Validated
mouse	WB	J Biol Chem; Ciliary neurotrophic factor (CNTF) protects retinal cone and rod photoreceptors by suppressing excessive formation of the visual pigments.; Authors - Songhua Li
pig	WB,IF	J Virol; Pemetrexed alleviates piglet diarrhea by blocking the interaction between porcine epidemic diarrhea virus nucleocapsid protein and Ezrin; Authors - Shujuan Zhang