Extra Range Prestained Protein Marker

Molecular Weight

10-310 kDa

Detection Method

Colorimetric, Fluorescence

Number of Markers

12

Stain Type

3 colors：Blue，Red，Green

Publications

3

Reviews (2)

Cat no : PL00003

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Validation Data Gallery

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  Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions
  The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions.

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  Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types as shown in figure.

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  The prestained protein ladders were resolved in an 8-16% Tris-glycine gel (SDS-PAGE). The image shows a comparison of the migration patterns on the gel for our standard (PL00001), broad (PL00002), and extra broad (PL00003) range ladders.

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  2 uL protein ladders were loaded in the 8%-18% Tris-glycine gel, then electrophoresed and transformed into the PVDF membrane. After blocking, this dual-channel image was taken directly by the Bio-Rad ChemiDoc MP Imaging System in the 550 nm (for 75 kDa and 310 kDa) and 680 nm (except 75 kDa and 310 kDa) range.

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Product Information

The PL00003 Extra Range Prestained Protein Marker is a ready-to-use three-color protein standard with 12 prestained proteins covering a wide range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer. The PL00003 Extra Range Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for estimating the size of proteins. The PL00003 Extra Range Prestained Protein Marker is also suitable for fluorescence WB detection.

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Storage

Store product at 4℃ for up to 12 weeks. For longer storage, aliquot and store at -20℃ for up to 1 year.

Usage

The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Detection Method

Colorimetric

Molecular Weight

~ 10, 15, 25, 35, 45, 60, 75, 100, 140, 180, 245, ~310 kDa

Number of Markers

12

Size Range

10 to 310

Cited in Article as

PL00003, Extra Range Prestained Protein Marker, Proteintech, IL, USA

Publications

Application	Title
	Cell Death Dis; EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli.; Authors - Shouyang Song
	CNS Neurosci Ther; A novel NMDA receptor modulator: the antidepressant effect and mechanism of GW043; Authors - Murezati Tiliwaerde
	Chin Med; Isoforskolin modulates AQP4-SPP1-PIK3C3 related pathway for chronic obstructive pulmonary disease via cAMP signaling; Authors - Haochang Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jakub (Verified Customer) (07-12-2024)

Excellent visibility of tested marker, even in lower amount than suggested. This product meets my expectations.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Brain - Rat

FH

Rocio (Verified Customer) (03-19-2024)

Works pretty well, all the colors are easy visible!

• Applications: Western Blot