Extra Range Prestained Protein Marker

Molecular Weight

10-310 kDa

Detection Method

Colorimetric, Fluorescence

Number of Markers


Stain Type

3 colors:Blue,Red,Green

Cat no : PL00003

Product Information

The PL00003 Extra Range Prestained Protein Marker is a ready-to-use three-color protein standard with 12 prestained proteins covering a wide range of molecular weights from 10 to 310 kDa in Tris-Glycine buffer. The PL00003 Extra Range Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for estimating the size of proteins. The PL00003 Extra Range Prestained Protein Marker is also suitable for fluorescence WB detection.

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).


Store product at 4℃ for up to 12 weeks. For longer storage, aliquot and store at -20℃ for up to 1 year.


The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Detection Method


Molecular Weight

~ 10, 15, 25, 35, 45, 60, 75, 100, 140, 180, 245, ~310 kDa

Number of Markers


Size Range

10 to 310

Cited in Article as

PL00003, Extra Range Prestained Protein Marker, Proteintech, IL, USA



Cell Death Dis

EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli.

Authors - Shouyang Song

CNS Neurosci Ther

A novel NMDA receptor modulator: the antidepressant effect and mechanism of GW043

Authors - Murezati Tiliwaerde

Chin Med

Isoforskolin modulates AQP4-SPP1-PIK3C3 related pathway for chronic obstructive pulmonary disease via cAMP signaling

Authors - Haochang Lin


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Rocio (Verified Customer) (03-19-2024)

Works pretty well, all the colors are easy visible!

  • Applications: Western Blot