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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Conjugate
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Exosome Expanded Antibody Kit

Host/Isotype

Rabbit/Mouse

Reactivity

Target dependent

Applications

Target dependent

Conjugate

Unconjugated

Cat no : PK30024

Validation Data Gallery

Applications:
  • Human urine and human urine-derived exosomes (human urine-exo) were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

    Human urine and human urine-derived exosomes (human urine-exo) were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Human plasma and human plasma-derived exosomes (human plasma-exo) were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

    Human plasma and human plasma-derived exosomes (human plasma-exo) were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Human urine and human urine-derived exosomes (human urine-exo) were subjected to SDS PAGE followed by western blot with 12422-1-AP (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    Human urine and human urine-derived exosomes (human urine-exo) were subjected to SDS PAGE followed by western blot with 12422-1-AP (Alix antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Human urine and human urine-derived exosomes (human urine-exo) were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    Human urine and human urine-derived exosomes (human urine-exo) were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

    human urine exosomes tissue were subjected to SDS PAGE followed by western blot with 25682-1-AP (CD63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Human urine and human urine-derived exosomes (human urine-exo) were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

    Human urine and human urine-derived exosomes (human urine-exo) were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Human urine and human urine-derived exosomes (human urine-exo) were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

    Human urine and human urine-derived exosomes (human urine-exo) were subjected to SDS PAGE followed by western blot with 82105-1-RR (CD9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 84142-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 84142-1-RR (CD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 66866-1-Ig (CD81 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

    Various lysates were subjected to SDS PAGE followed by western blot with 67381-1-Ig (TSG101 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Various cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

    Various cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 66316-1-Ig (EPCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB result of EPCAM antibody (66316-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells.

    WB result of EPCAM antibody (66316-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells.

  • Various lysates were subjected to SDS PAGE followed by western blot with 60318-1-Ig (HSP90 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

    Various lysates were subjected to SDS PAGE followed by western blot with 60318-1-Ig (HSP90 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Various lysates were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 10995-1-AP (HSP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 66662-1-Ig (GOLGA2/GM130 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells.

    WB result of GOLGA2/GM130 antibody (66662-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HEK-293 cells.

  • Various lysates were subjected to SDS PAGE followed by western blot with 12422-1-AP (Alix antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

    Various lysates were subjected to SDS PAGE followed by western blot with 12422-1-AP (Alix antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

Product Information

The Exosome Essentials Antibody Kit provides a cost-effective tool for identifying and studying exosomal markers. Perfect for researchers starting a new project, screening multiple prospective targets or those who simply require less volume. 

The Exosome Expanded Antibody Kit contains antibodies against 10 key proteins for studying exosomes (including anti-GM-130 as a negative control). 


Antigen Catalog No. Host, clonality Tested Reactivity Applications Volume

CD63

25682-1-AP             Rabbit Polyclonal             H WB, IP, IF, IHC 20 uL

Alix

12422-1-AP             Rabbit Polyclonal             H, M, R WB, IP, IF, IHC 20 uL

TSG101

67381-1-Ig             Mouse monoclonal  H, M, R WB, IF, IHC 20 uL

HSP70

10995-1-AP             Rabbit Polyclonal             H, M, R WB, IP, IF, IHC 20 uL

GOLGA2/GM130

66662-1-Ig             Mouse monoclonal  H, R WB, IF  20 uL            
Annexin V 66245-1-Ig
            		Mouse monoclonal              H, M      WB, IF, IHC  20 uL            
EpCAM 66316-1-Ig
            		Mouse monoclonal              H      WB, IF, IHC          20 uL            
HSP90 60318-1-Ig
            		Mouse monoclonal              H, M, R, Pg              WB, IP, IF, IHC             20 uL            
CD9 84142-1-RR
            		Rabbit monoclonal             H         WB, IF, IHC             20 uL            
CD81 66866-1-Ig
            		Mouse monoclonal            H             WB, IF, IHC          20 uL            


Also see our 'Exosome Essentials Antibody Kit ' on the following page  https://www.ptglab.com/products/Exosome-Essentials-Antibody-Kit-PK30023.htm。

Storage

Store at -20°C. Stable for one year from the date of receipt. 

Background Information

Exosomes are small, membrane-bound vesicles that are secreted by various types of cells in normal and pathophysiological conditions, including cancers, neurodegenerative disorders, and cardiovascular diseases. They are involved in intercellular communication and are responsible for the transfer of bioactive molecules, such as nuclic acids, proteins, and lipids. In addition to analyzing cargo from isolated exosomes, scientists have also been harnessing the potential of exosomes for drug delivery and regenerative medicine.  

Standard Protocols

Click   here  to view our standard protocols for various applications including WB, IP, IHC, IF, FC, and ELISA.

Cited in Article as

PK30024, Exosome Expanded Antibody Kit, Proteintech, IL, USA