Na Estradiol Monoclonal antibody, PBS Only

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  ELISA experiment of Estradiol using 68600-1-Ig (same clone as 68600-1-PBS)

  Indirect ELISA was performed by coating BSA-Estradiol ~30 ng/well (by Estradiol amount), followed by blocking with 1% BSA. Serial diluted Estradiol antibody 68600-1-Ig was added to the plates and incubated at 37℃. HRP-Goat anti-mouse was used for detection (top X-right Y, blue curve). Competitive ELISA was performed similarly except that different concentration of Estradiol was mixed in 30ng/mL primary antibody (bottom X-left Y, red curve). This data was developed using the same antibody clone with 68600-1-PBS in a different storage buffer formulation.

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  ELISA experiment of Estradiol using 68600-1-PBS

  BSA-Estradiol was coated in 96 well plate at ~30 ng/well (by Estradiol amount), followed by blocking with 1% BSA. Different concentrations of Estradiol standard as well as diluted urine from different donor were mixed with 30 ng/mL Estradiol antibody 68600-1-Ig respectively. Urine Estradiol was calculated based on standard curve. Please notice that the level from different donor might be different between individuals. This data was developed using the same antibody clone with 68600-1-PBS in a different storage buffer formulation.

Estradiol Monoclonal Antibody for ELISA, Indirect ELISA

Cat No. 68600-1-PBS

Clone No.2E5E4

Host / Isotype

Mouse / IgG1

Reactivity

Estradiol

Applications

ELISA, Indirect ELISA

Synonyms

17 beta oestradiol, beta Estradiol, C18H24O2, cis-Estradiol, Estradiol, Estradiol hemihydrate, Estradiolum

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Product Information

68600-1-PBS targets Estradiol in ELISA, Indirect ELISA applications and shows reactivity with Estradiol samples.

• Tested Reactivity: Estradiol
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PTG
• Full Name: Estradiol
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Estradiol is a naturally occurring hormone circulating endogenously in females. It is commercially available in several hormone therapy products for managing conditions associated with reduced estrogen, such as vulvovaginal atrophy and hot flashes. Some available forms of estradiol include oral tablets, injections, vaginal rings, transdermal patches, sprays, gels, and creams. This antibody can be used in the Elisa experiments.