EPCAM/CD326 Recombinant antibody, PBS Only (Detector)

EPCAM/CD326 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241243E4

Cat no : 84073-4-PBS

Synonyms

EPCAM, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1, CD326

---

Validation Data Gallery

• 

  Sandwich ELISA standard curve of MP01009-4

  Sandwich ELISA standard curve of MP01009-4, Human EPCAM/CD326 Recombinant Matched Antibody Pair - PBS only. 84073-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1370. 84073-4-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

• 

  Sample test of MP01009-4

  The mean EPCAM/CD326 concentration was determined to be 492922.2 pg/mL in HT-116 cell extract based on a 2.0 mg/mL extract load.

• 

  Sample test of MP01009-4

  HT-29 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human EPCAM/CD326, and measured 6718.5 pg/mL.

• 

  Sample test of MP01009-4

  Serum of six individual healthy human donors was measured. The EPCAM/CD326 concentration of detected samples was determined to be 484.9 pg/mL with a range of 81.5-2002.2 pg/mL.

• 

  Cytometric bead array standard curve of MP01009-2

  Cytometric bead array standard curve of MP01009-2, EPCAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84073-5-PBS. Detection antibody: 84073-4-PBS. Standard: Eg1370. Range: 0.156-20 ng/mL.

---

Product Information

84073-4-PBS targets EPCAM/CD326 as part of a matched antibody pair:

MP01009-2: 84073-5-PBS capture and 84073-4-PBS detection (validated in Cytometric bead array)

MP01009-4: 84073-7-PBS capture and 84073-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: epithelial cell adhesion molecule
• Calculated Molecular Weight: 35 kDa
• GenBank Accession Number: NM_002354.3
• Gene Symbol: EPCAM
• Gene ID (NCBI): 4072
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.