Endoglin/CD105 Recombinant antibody

Endoglin/CD105 Uni-rAb^TM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230047E8

Cat no : 82993-5-RR

Synonyms

CD105, END, Endoglin, ENG, HHT1, ORW, ORW1, 230047E8

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Validation Data Gallery

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  IF Staining of HUVEC using 82993-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ENG antibody (82993-5-RR, Clone: 230047E8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  Affinity and Kinetic Characterization of 82993-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82993-5-RR against Human Endoglin/CD105 were performed. The affinity constant is 0.296 nM.

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Tested Applications

• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82993-5-RR is Endoglin/CD105 Fusion Protein expressed in E. coli.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: endoglin
• Calculated Molecular Weight: 71 kDa
• GenBank Accession Number: BC014271
• Gene Symbol: CD105
• Gene ID (NCBI): 2022
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)