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WB analysis using 67075-1-Ig (same clone as 67075-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
WB analysis of HL-60 using 67075-1-Ig (same clone as 67075-1-PBS)
HL-60 cells were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
WB analysis of HUVEC using 67075-1-Ig (same clone as 67075-1-PBS)
HUVEC cells were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 67075-1-Ig (same clone as 67075-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67075-1-Ig (same clone as 67075-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IHC staining of human breast cancer using 67075-1-Ig (same clone as 67075-1-PBS)
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 67075-1-Ig (same clone as 67075-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 67075-1-Ig (same clone as 67075-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IHC staining of human placenta using 67075-1-Ig (same clone as 67075-1-PBS)
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 67075-1-Ig (same clone as 67075-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IHC staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IHC staining of human renal cell carcinoma using 67075-1-Ig (same clone as 67075-1-PBS)
Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:10000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IF Staining of human tonsillitis using 67075-1-Ig (same clone as 67075-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:10000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IF Staining of human liver cancer using 67075-1-Ig (same clone as 67075-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IF Staining of human liver cancer using 67075-1-Ig (same clone as 67075-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
IF Staining of human placenta using 67075-1-Ig (same clone as 67075-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.
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Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67075-1-PBS in a different storage buffer formulation.

Endoglin/CD105 Monoclonal antibody, PBS Only

Endoglin/CD105 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Cat No. 67075-1-PBS

Clone No.2D5E8

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

CD105, 2D5E8, HHT1, ENG, Endoglin

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67075-1-PBS targets Endoglin/CD105 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	Endoglin/CD105 fusion protein Ag27837 Predict reactive species
Full Name	endoglin
Calculated Molecular Weight	71 kDa
Observed Molecular Weight	95-100 kDa
GenBank Accession Number	BC014271
Gene Symbol	CD105
Gene ID (NCBI)	2022
RRID	AB_2882383
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P17813
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)