Egfr Polyclonal antibody

Egfr Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30847-1-AP

Synonyms

9030024J15Rik, AI552599, Egfr, Erbb, Errp, wa 2, wa2, Wa5



Tested Applications

Positive WB detected inA549 cells, mouse liver tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30847-1-AP targets Egfr in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Egfr fusion protein Ag33621
Full Name epidermal growth factor receptor
Calculated Molecular Weight135 kDa
Observed Molecular Weight140-180 kDa
GenBank Accession NumberNM_207655
Gene Symbol Egfr
Gene ID (NCBI) 13649
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The epidermal growth factor receptor (EGFR) is a member of the ErbB family of receptor tyrosine kinases (RTKs). Binding of EGFR to epidermal growth factor family ligands triggers a signaling cascade through allosteric activation of epidermal growth factor receptor kinase and trans-autophosphorylation of key tyrosine residues in the tail of the cytoplasmic receptor (PMID: 24691965). Due to phosphorylation, the apparent molecular weight is higher than the calculated molecular weight of 140 kDa. EFGR has been implicated in learning and memory enhancement and pain signaling in mammals (PMID: 20639532 and 35131940).

Protocols

Product Specific Protocols
WB protocol for Egfr antibody 30847-1-APDownload protocol
IF protocol for Egfr antibody 30847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols