EGFR Polyclonal antibody

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  WB analysis using 30847-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30847-1-AP (Egfr antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 30847-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Egfr antibody (30847-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

EGFR Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30847-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

HER1, Errp, Erbb1, erbb 1, Erbb

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Tested Applications

• Positive WB detected in: A549 cells, mouse liver tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30847-1-AP targets EGFR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EGFR fusion protein Ag33621
• Full Name: epidermal growth factor receptor
• Calculated Molecular Weight: 135 kDa
• Observed Molecular Weight: 140-180 kDa
• GenBank Accession Number: NM_207655
• Gene Symbol: Egfr
• Gene ID (NCBI): 13649
• RRID: AB_3086421
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q01279
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The epidermal growth factor receptor (EGFR) is a member of the ErbB family of receptor tyrosine kinases (RTKs). Binding of EGFR to epidermal growth factor family ligands triggers a signaling cascade through allosteric activation of epidermal growth factor receptor kinase and trans-autophosphorylation of key tyrosine residues in the tail of the cytoplasmic receptor (PMID: 24691965). Due to phosphorylation, the apparent molecular weight is higher than the calculated molecular weight of 140 kDa. EFGR has been implicated in learning and memory enhancement and pain signaling in mammals (PMID: 20639532 and 35131940).