EZH1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EZH1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 20852-1-AP

Synonyms

Enhancer of zeste homolog 1, ENX 2, EZH1, KIAA0388

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Validation Data Gallery

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  WB analysis of RAW 264.7 using 20852-1-AP

  WB result of EZH1 antibody (20852-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EZH1 transfected RAW 264.7 cells.

• 

  WB analysis of HL-60 using 20852-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20852-1-AP (EZH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20852-1-AP

  HEK-293T cell lysates were subjected to SDS PAGE followed by western blot with 20852-1-AP (EZH1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: RAW 264.7 cells, HL-60 cells, HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:300-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 1 publications below

Product Information

The immunogen of 20852-1-AP is EZH1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EZH1 fusion protein Ag14732
• Full Name: enhancer of zeste homolog 1 (Drosophila)
• Calculated Molecular Weight: 747 aa, 85 kDa
• Observed Molecular Weight: 80-95 kDa
• GenBank Accession Number: BC015882
• Gene Symbol: EZH1
• Gene ID (NCBI): 2145
• RRID: AB_10863659
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
human,chicken	WB,IF	Nat Commun; Gain and loss of function variants in EZH1 disrupt neurogenesis and cause dominant and recessive neurodevelopmental disorders; Authors - Carolina Gracia-Diaz
mouse	WB	Oxid Med Cell Longev; The Dietary Supplement γ-Oryzanol Attenuates Hepatic Ischemia Reperfusion Injury via Inhibiting Endoplasmic Reticulum Stress and HMGB1/NLRP3 Inflammasome.; Authors - Yichao Du
human	WB	Eur J Med Chem; Discovery of precision targeting EZH2 degraders for triple-negative breast cancer.; Authors - Cheng Wang
mouse	WB	Drug Discov Ther; Ezh1 regulates expression of Cpg15/Neuritin in mouse cortical neurons.; Authors - Shun Utsunomiya ; KO Validated
human	WB	Photochem Photobiol; The m6 A reader YTHDC2 regulates UVB-induced DNA damage repair and histone modification; Authors - Zizhao Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mengxue (Verified Customer) (11-29-2018)

This is the 5th EZH1 Ab I've tried so far and the only one that works with our rat primary cells. I got clear band on Western and I'm so happy about it!

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: rat primary fibroblast cells