EYA1 Polyclonal antibody

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  WB analysis using 31435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31435-1-AP (EYA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

EYA1 Polyclonal Antibody for WB, ELISA

Cat No. 31435-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Synonyms

BOP, BOR, EYA1, Eyes absent homolog 1

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Tested Applications

• Positive WB detected in: NCCIT cells, mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31435-1-AP targets EYA1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EYA1 fusion protein Ag33438
• Full Name: eyes absent homolog 1 (Drosophila)
• Calculated Molecular Weight: 592 aa, 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC121799
• Gene Symbol: EYA1
• Gene ID (NCBI): 2138
• RRID: AB_3669982
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q99502
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The EYA1 (Eyes absent homolog 1) gene is the human homologue of the Drosophila Eya1 gene, which is essential for eye development in that species. There are 4 different isoforms of EYA1 (EYA1A, EYA1B, EYA1C, EYA1D), which are composed of 559 amino acids (AA), 592 AA, 592 AA and 557 AA, respectively. It should be noted that isoforms B and C have the same length; however, they could be considered different variants because of change in amino acidic composition (PMID: 24803398).