EXOSC6 Recombinant antibody

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  IF Staining of HeLa using 84346-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EXOSC6 antibody (84346-2-RR, Clone: 241535G9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84346-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84346-2-RR against Human EXOSC6 were performed. The affinity constant is 0.399 nM.

EXOSC6 {ptg:Brand} Recombinant Antibody for IF/ICC, ELISA

Cat No. 84346-2-RR

Clone No.241535G9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

MTR3, hMtr3p, hMtr3, exosome component 6, EAP4

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84346-2-RR targets EXOSC6 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: EXOSC6 fusion protein Ag33513
• Full Name: exosome component 6
• Calculated Molecular Weight: 28kd
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: NM_058219
• Gene Symbol: EXOSC6
• Gene ID (NCBI): 118460
• RRID: AB_3671886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5RKV6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOSC6 (exosome component 6), also known as p11 or MTR3. The calculated molecular weight of EXOSC6 is 28 kDa. And it has low tissue specificity. The gene product constitutes one of the subunits of the multisubunit particle called exosome, which mediates mRNA degradation. It is also a component of the RNA exosome complex (PMID: 29906447). The composition of human exosome is similar to its yeast counterpart. EXOSC6 is homologous to the yeast Mtr3 protein. Its exact function is not known, however, it has been shown using a cell-free RNA decay system that the exosome is required for rapid degradation of unstable mRNAs containing AU-rich elements (AREs), but not for poly(A) shortening. The exosome does not recognize ARE-containing mRNAs on its own, but requires ARE-binding proteins that could interact with the exosome and recruit it to unstable mRNAs, thereby promoting their rapid degradation.