EXOSC5 Polyclonal antibody

EXOSC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (2)

Cat no : 15627-1-AP

Synonyms

Ribosomal RNA-processing protein 46, p12B, hRrp46p, Exosome component 5, Exosome complex component RRP46

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Validation Data Gallery

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  WB analysis using 15627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15627-1-AP (EXOSC5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15627-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15627-1-AP (EXOSC5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15627-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15627-1-AP (EXOSC5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15627-1-AP

  IP result of anti-EXOSC5 (IP:15627-1-AP, 4ug; Detection:15627-1-AP 1:300) with HeLa cells lysate 2800 ug.

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  IHC staining of human breast cancer using 15627-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15627-1-AP (EXOSC5 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 15627-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 15627-1-AP (EXOSC5 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 15627-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using EXOSC5 antibody (15627-1-AP) at dilution of 1:700 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Raji cells, HeLa cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:350-1:1400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

15627-1-AP targets EXOSC5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXOSC5 fusion protein Ag8098
• Full Name: exosome component 5
• Calculated Molecular Weight: 235 aa, 25 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC007742
• Gene Symbol: EXOSC5
• Gene ID (NCBI): 56915
• RRID: AB_2293809
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CML28 is a component of the human exosome, which is a multiprotein complex involved in the 3′ processing and degradation of RNA [PMID:12359762]. CML28 is a novel 28 kDa TAA identified by serological analysis of cDNA expression libraries (SEREX) screening of a chronic myeloid leukemia (CML) cDNA expression library. As a potential target for tumor-specific immunotherapy, CML28 is superior to other known TAAs characterized by its broader spectrum expression in tumor cells and stricter expression in normal tissues [PMID:16815301].

Publications

Species	Application	Title
human	WB,IHC	J Cancer; EXOSC5 promotes proliferation of gastric cancer through regulating AKT/STAT3 signaling pathways.; Authors - Xiangliu Chen
human	IHC	Int J Mol Sci; Integrated Bioinformatic Investigation of EXOSCs in Hepatocellular Carcinoma Followed by the Preliminary Validation of EXOSC5 in Cell Proliferation; Authors - Yujing Zhang
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (07-06-2022)

Reduction of Exosc5 is observed when comparing depletion vs control. Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESCs

EXOSC5 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:15627-1-AP)

FH

Nikolaus (Verified Customer) (05-24-2022)

Depletion is a conditional KO time-course; strong cross-reactivity with Bio-Rad dual stain ladder (25 kDa band)

• Applications: Western Blot
• Primary Antibody Dilution: 1/2000
• Cell Tissue Type: mESC

EXOSC5 Antibody Western Blot validation (1/2000 dilution) in mESC (Cat no:15627-1-AP)