EXOSC2 Monoclonal antibody, PBS Only

EXOSC2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G8B1

Cat no : 66099-1-PBS

Synonyms

EXOSC2, exosome component 2, hRrp4p, p7, RRP4, Rrp4p

---

Validation Data Gallery

• 

  WB analysis using 66099-1-Ig (same clone as 66099-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66099-1-Ig (same clone as 66099-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66099-1-Ig (same clone as 66099-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66099-1-Ig (same clone as 66099-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66099-1-Ig (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66099-1-Ig (same clone as 66099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66099-1-Ig (same clone as 66099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 66099-1-Ig (same clone as 66099-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin cancer slide using 66099-1-Ig (EXOSC2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66099-1-Ig (same clone as 66099-1-PBS)

  Immunofluorescent analysis of () fixed HepG2 cells using 66099-1-Ig (EXOSC2 antibody) at dilution of 1:25. This data was developed using the same antibody clone with 66099-1-PBS in a different storage buffer formulation.

---

Product Information

66099-1-PBS targets EXOSC2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: EXOSC2 fusion protein Ag7003
• Full Name: exosome component 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC000747
• Gene Symbol: EXOSC2
• Gene ID (NCBI): 23404
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].