EXOSC2 Polyclonal antibody

• Featured Product
• KD/KO Validated

EXOSC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (2)

Cat no : 14805-1-AP

Synonyms

Rrp4p, RRP4, Ribosomal RNA-processing protein 4, hRrp4p, Exosome component 2

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Validation Data Gallery

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  WB analysis of HEK-293 using 14805-1-AP

  WB result of EXOSC2 antibody (14805-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC2 transfected HEK-293 cells.

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  WB analysis of HeLa using 14805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14805-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14805-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14805-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14805-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14805-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14805-1-AP (EXOSC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14805-1-AP

  IP result of anti-EXOSC2 (IP:14805-1-AP, 4ug; Detection:14805-1-AP 1:1000) with HeLa cells lysate 1080ug.

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  IHC staining of human skin cancer using 14805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14805-1-AP (EXOSC2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin cancer using 14805-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 14805-1-AP (EXOSC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of MCF-7 using 14805-1-AP

  Immunofluorescent analysis of MCF-7 cells, using EXOSC2 antibody 14805-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, HEK-293 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Product Information

14805-1-AP targets EXOSC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXOSC2 fusion protein Ag6525
• Full Name: exosome component 2
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-33 kDa
• GenBank Accession Number: BC000747
• Gene Symbol: EXOSC2
• Gene ID (NCBI): 23404
• RRID: AB_2101837
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snoRNA and snRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs [PMID:15346807]. EXOSC2 is a non-catalytic component of the RNA exosome complex that has 3'->5' exoribonuclease activity and involves in a multitude of cellular RNA processing and degradation events [PMID: 17545563].

Publications

Species	Application	Title
human	WB,IF	Cell; Modulated Expression of Specific tRNAs Drives Gene Expression and Cancer Progression.; Authors - Hani Goodarzi
human		Cell; The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.; Authors - Jakob Trendel
human	WB	Life Sci Alliance; Low expression of EXOSC2 protects against clinical COVID-19 and impedes SARS-CoV-2 replication; Authors - Tobias Moll
human	WB	Mol Biotechnol; EXOSC2 Mediates the Pro-tumor Role of WTAP in Breast Cancer Cells via Activating the Wnt/β-Catenin Signal; Authors - Chen-Guang Lv ; KD Validated
human,mouse	WB	Mol Cell; The MYCN oncoprotein is an RNA-binding accessory factor of the nuclear exosome targeting complex; Authors - Dimitrios Papadopoulos

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tsimafei (Verified Customer) (12-04-2022)

50µg of total protein were loaded on a gel. Incubation ON at 4C

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mESCs

EXOSC2 Antibody Western Blot validation (1:5000 dilution) in mESCs (Cat no:14805-1-AP)

FH

Nikolaus (Verified Customer) (07-14-2022)

Antibodies were incubated ON at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mESCs

EXOSC2 Antibody Western Blot validation (1:1000 dilution) in mESCs (Cat no:14805-1-AP)