EXO1 Polyclonal antibody

• Featured Product
• KD/KO Validated

EXO1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 16253-1-AP

Synonyms

EXO1, EXOI, exonuclease 1, Exonuclease I, HEX1, hExo1, hExoI

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Validation Data Gallery

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  WB analysis using 16253-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16253-1-AP (EXO1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16253-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16253-1-AP (EXO1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 16253-1-AP

  IP result of anti-EXO1 (IP:16253-1-AP, 4ug; Detection:16253-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

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Tested Applications

• Positive WB detected in: HEK-293T cells, Jurkat cells, HeLa cells, Raji cells
• Positive IP detected in: mouse thymus tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• ChIP: See 1 publications below

Product Information

16253-1-AP targets EXO1 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EXO1 fusion protein Ag9253
• Full Name: exonuclease 1
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 115-120 kDa
• GenBank Accession Number: BC007491
• Gene Symbol: EXO1
• Gene ID (NCBI): 9156
• RRID: AB_2278140
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXO1, also named as HEX1, is a 846 amino acid protein, which belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. EXO1 is highly expressed in bone marrow, testis and thymus and at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. EXO1 as 5'->3' double-stranded DNA exonuclease, which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. EXO1 functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. The calculated molecular weight of EXO1 is 94 kDa, but the modified EXO1 protein is about 115 kDa.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; DNA double-strand breaks with 5' adducts are efficiently channeled to the DNA2-mediated resection pathway.; Authors - Margaret Tammaro
human	WB	Nucleic Acids Res; PCNA promotes processive DNA end resection by Exo1.; Authors - Chen Xiaoqing X ; KD Validated
human	WB	Elife; Perturbation of base excision repair sensitizes breast cancer cells to APOBEC3 deaminase-mediated mutations.; Authors - Birong Shen ; KD Validated
human	WB	PLoS Genet; EEPD1 Rescues Stressed Replication Forks and Maintains Genome Stability by Promoting End Resection and Homologous Recombination Repair.; Authors - Yuehan Wu
rat	WB	J Cardiovasc Transl Res; Exosomes Derived from AT2R-Overexpressing BMSC Prevent Restenosis After Carotid Artery Injury by Attenuating the Injury-Induced Neointimal Hyperplasia.; Authors - Xinliang Zou
human	WB	Front Genet; Telomeric Double Strand Breaks in G1 Human Cells Facilitate Formation of 5' C-Rich Overhangs and Recruitment of TERRA.; Authors - Christopher B Nelson ; KD Validated