mouse lung tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 20138-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20138-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse lung using 20138-1-AP
IP result of anti-EXD2 (IP:20138-1-AP, 3ug; Detection:20138-1-AP 1:300) with mouse lung tissue lysate 5160ug.
Immunohistochemical analysis of paraffin-embedded human testis using 20138-1-AP (EXD2 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HepG2 using 20138-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EXD2 antibody (20138-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EXD2 antibody (20138-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:1000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
mouse lung tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 20138-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 20138-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 20138-1-AP (EXD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human testis using 20138-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 20138-1-AP (EXD2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 20138-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 20138-1-AP (EXD2 antibody) at dilution of 1:50 (under 40x lens).
IP Figures
IP experiment of mouse lung using 20138-1-AP
IP result of anti-EXD2 (IP:20138-1-AP, 3ug; Detection:20138-1-AP 1:300) with mouse lung tissue lysate 5160ug.
IF/ICC Figures
IF Staining of HepG2 using 20138-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EXD2 antibody (20138-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
EXD2 Polyclonal antibody
Catalog Number
20138-1-AP
Citations
2
Dilutions
WB : 1:500-1:1000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:50-1:500
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
