EVL Polyclonal antibody, PBS Only

EVL Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13484-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of Jurkat using 13484-1-AP (same clone as 13484-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13484-1-AP (EVL antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 13484-1-AP (same clone as 13484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human small intestine using 13484-1-AP (same clone as 13484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 13484-1-AP (same clone as 13484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 13484-1-AP (same clone as 13484-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 13484-1-AP (EVL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13484-1-PBS in a different storage buffer formulation.

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Product Information

13484-1-PBS targets EVL in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EVL fusion protein Ag4302
• Full Name: Enah/Vasp-like
• Calculated Molecular Weight: 418 aa, 45 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC032358
• Gene Symbol: EVL
• Gene ID (NCBI): 51466
• RRID: AB_10644121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

EVL (Ena/VASP-like protein) is implicated in processes that requires dynamic actin remodeling such as axon guidance and platelet activation.It has two isoforms, EVL and EVL-I,that are highly expressed in hematopoietic cells of thymus and spleen,and both isoforms can be phosphorylated by PKA in vitro(PMID10945997).