EVI1 Monoclonal antibody

EVI1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A12A6

Cat no : 60413-1-Ig

Synonyms

MDS1 EVI1, EVI-1, EVI 1, EC:2.1.1.367, AML1 EVI 1

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Validation Data Gallery

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  WB analysis of MKN-45 using 60413-1-Ig

  MKN-45 cells were subjected to SDS PAGE followed by western blot with 60413-1-Ig (EVI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 60413-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60413-1-Ig (EVI1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MKN-45 cells, Calu-3 cells, MDA-MB-231 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60413-1-Ig targets EVI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: EVI1 fusion protein Ag34143
• Full Name: ecotropic viral integration site 1
• Calculated Molecular Weight: 138 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: NM_001105077
• Gene Symbol: EVI1
• Gene ID (NCBI): 2122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.