ETS1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ETS1 Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F2D4

Cat no : 66598-1-PBS

Synonyms

ETS 1, ETS1, EWSR2, p54, Protein C ets 1

---

Validation Data Gallery

• 

  WB analysis of HepG2 using 66598-1-Ig (same clone as 66598-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66598-1-Ig (same clone as 66598-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Daudi using 66598-1-Ig (same clone as 66598-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 66598-1-Ig (same clone as 66598-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66598-1-Ig (ETS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 66598-1-Ig (same clone as 66598-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (66598-1-Ig, Clone: 4F2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66598-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66598-1-PBS targets ETS1 in WB, IF, Indirect ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ETS1 fusion protein Ag2127
• Full Name: v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
• Calculated Molecular Weight: 441 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC017314
• Gene Symbol: ETS1
• Gene ID (NCBI): 2113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo.