ETS1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

ETS1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12118-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 12118-1-AP (same clone as 12118-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12118-1-AP (ETS1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 12118-1-AP (same clone as 12118-1-PBS)

  WB result of ETS1 antibody (12118-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ETS1 transfected Jurkat cells. This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 12118-1-AP (same clone as 12118-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12118-1-AP (ETS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 12118-1-AP (same clone as 12118-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12118-1-AP (ETS1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat spleen using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat lung using 12118-1-AP (same clone as 12118-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 12118-1-AP (ETS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 12118-1-AP (same clone as 12118-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using ETS1 antibody (12118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12118-1-PBS in a different storage buffer formulation.

---

Product Information

12118-1-PBS targets ETS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ETS1 fusion protein Ag2127
• Full Name: v-ets erythroblastosis virus E26 oncogene homolog 1 (avian)
• Calculated Molecular Weight: 441 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC017314
• Gene Symbol: ETS1
• Gene ID (NCBI): 2113
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ETS1 is a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. ETS1 is a nuclear protein and primarily acts as a transcriptional activator, though it can also repress gene transcription. Since it is primarily expressed in lymphocytes, it plays several critical roles in immunity. In addition, it is important for angiogenesis. Since cells express several Ets proteins at the same time, functional redundancy is possible. This is particularly shown for the socalled housekeeping genes where several Ets factors were found to occupy the proximal promoters in vivo. The ETS1 protein exists as three isoforms: ETS1-p51, ETS1-p42, ETS1-p27 ranging in size from 27 to 51 kDa.