mouse brain tissue were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 67661-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 67661-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 67661-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67661-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 67661-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ETFA antibody (67661-1-Ig, Clone: 3C12G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue).
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HCT 116 cells, LNCaP cells, mouse brain tissue, HuH-7 cells, HeLa cells, K-562 cells, pig liver tissue, rat liver tissue
Positive IHC detected in
human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:8000-1:32000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
67661-1-Ig targets ETFA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The electron transfer flavoprotein(ETF), located in the mitochondrial matrix, is a nuclear-encoded enzyme delivering to the respiratory chain by straight-chain acyl-CoA dehydrogenases and other dehydrogenases. Mitochondrial fatty acids oxidation and amino acids metabolism are affected in MADD(Multiple acyl-CoA dehydrogenase deficiency). These disorders are linked to a dysfunction of ETF. ETF is a mitochondrial matrix protein consisting of alpha- (30kDa) and beta- (28kDa) subunits encoded by the ETFA and ETFB genes, respectively. (PMID: 31996215,PMID: 16510302)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 67661-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 67661-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67661-1-Ig (ETFA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human liver cancer using 67661-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 67661-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67661-1-Ig (ETFA antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 67661-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ETFA antibody (67661-1-Ig, Clone: 3C12G5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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