ETF1 Polyclonal antibody

ETF1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10884-1-AP

Synonyms

TB3-1, TB3 1, SUP45L1, RF1, ERF1

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Validation Data Gallery

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  WB analysis using 10884-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10884-1-AP (ETF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10884-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10884-1-AP (ETF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10884-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10884-1-AP (ETF1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 10884-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10884-1-AP (ETF1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10884-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10884-1-AP (ETF1 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10884-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ETF1 antibody (10884-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: T-47D cells, mouse testis tissue, SKOV-3 cells
• Positive IHC detected in: human pancreas cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

10884-1-AP targets ETF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ETF1 fusion protein Ag1330
• Full Name: eukaryotic translation termination factor 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC014269
• Gene Symbol: ETF1
• Gene ID (NCBI): 2107
• RRID: AB_2100536
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome, which is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In eukaryotes a single class-1 translation termination factor eRF1 decodes the three stop codons: UAA, UAG and UGA. [PMID:7990965,20860996]

Publications

Species	Application	Title
human	IF	Cells; Relocalization of Translation Termination and Ribosome Recycling Factors to Stress Granules Coincides with Elevated Stop-Codon Readthrough and Reinitiation Rates upon Oxidative Stress; Authors - Desislava S Makeeva