ESYT2 Monoclonal antibody

• Featured Product
• KD/KO Validated

ESYT2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B12G2

Cat no : 68374-1-Ig

Synonyms

CHR2SYT, E Syt2, ESYT2, Extended synaptotagmin 2, FAM62B, KIAA1228

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Validation Data Gallery

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  WB analysis using 68374-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (FAM62B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68374-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68374-1-Ig (ESYT2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68374-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68374-1-Ig (FAM62B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 68374-1-Ig

  WB result of FAM62B antibody (68374-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAM62B transfected HeLa cells.

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Tested Applications

• Positive WB detected in: HeLa cells, A549 cells, U2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68374-1-Ig targets ESYT2 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ESYT2 fusion protein Ag20440
• Full Name: family with sequence similarity 62 (C2 domain containing) member B
• Calculated Molecular Weight: 921 aa, 102 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC013957
• Gene Symbol: ESYT2
• Gene ID (NCBI): 57488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ESYT2, also named FAM62B, is one of the three-member family of Extended Synaptotagmins that were recently shown to be implicated in the formation of endoplasmic reticulum (ER)-plasma membrane (PM) junctions and in the Ca(2+) dependent regulation of these junctions.