ESRP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ESRP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 21045-1-AP

Synonyms

RNA-binding protein 35A, RNA-binding motif protein 35A, RNA binding protein 35A, RNA binding motif protein 35A, RBM35A

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Validation Data Gallery

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  WB analysis of MCF-7 using 21045-1-AP

  WB result of ESRP1 antibody (21045-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ESRP1 transfected MCF-7 cells.

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  WB analysis of COLO 320 using 21045-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 21045-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21045-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 21045-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21045-1-AP (ESRP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 21045-1-AP

  IP result of anti-ESRP1 (IP:21045-1-AP, 4ug; Detection:21045-1-AP 1:300) with COLO 320 cells lysate 4000ug.

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  IHC staining of human lung using 21045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung using 21045-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 21045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21045-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human bowen disease using 21045-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 21045-1-AP (ESRP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells, mouse lung tissue, MCF-7 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human lung tissue, mouse colon tissue, human bowen disease; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• RIP: See 1 publications below

Product Information

21045-1-AP targets ESRP1 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ESRP1 fusion protein Ag15386
• Full Name: epithelial splicing regulatory protein 1
• Calculated Molecular Weight: 681 aa, 76 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC099916
• Gene Symbol: ESRP1
• Gene ID (NCBI): 54845
• RRID: AB_2878801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ESRP1, also named as Epithelial splicing regulatory protein 1, is a 681 amino acid protein, which belongs to the ESRP family. ESRP1 as a mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. ESRP1 specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2 and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). ESRP1 exists various isoforms and molecular weight of each isoform is 76 kDa, 67 kDa, and 73 kDa.

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; ESRP1 controls biogenesis and function of a large abundant multiexon circRNA; Authors - Dawei Liu ; KD Validated
	RIP	Mol Ther; circCAMSAP1 Promotes Tumor Growth in Colorectal Cancer via the miR-328-5p/E2F1 Axis.; Authors - Chi Zhou ; KD Validated
human	WB	Cell Death Differ; Splicing factor derived circular RNA circUHRF1 accelerates oral squamous cell carcinoma tumorigenesis via feedback loop.; Authors - Wei Zhao
human		Oncogene; Androgen receptor signaling regulates the transcriptome of prostate cancer cells by modulating global alternative splicing.; Authors - Kalpit Shah
human	WB	Elife; Transcriptional control of subtype switching ensures adaptation and growth of pancreatic cancer.; Authors - Christina R Adams
human	WB,IF	Cell Signal; Silencing ESRP1 expression promotes caspase-independent cell death via nuclear translocation of AIF in colon cancer cells.; Authors - Yellamandayya Vadlamudi ; KD Validated