ESR2 Beta 6 Polyclonal antibody

ESR2 Beta 6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55471-1-AP

Synonyms

ER BETA, Erb, ESR BETA, ESR2, ESR2 Beta 6, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2

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Validation Data Gallery

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  WB analysis of mouse spleen using 55471-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 55471-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 55471-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervix using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 55471-1-AP (ESR2 Beta-6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 55471-1-AP (ESR2 Beta-6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 55471-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55471-1-AP (ESR2 Beta-6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 55471-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ESR2 Beta 6 antibody (55471-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red), DAPI (Blue).

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Tested Applications

• Positive WB detected in: mouse spleen tissue
• Positive IHC detected in: human prostate cancer tissue, human cervix tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

55471-1-AP targets ESR2 Beta 6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: estrogen receptor 2 (ER beta)
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001437
• Gene Symbol: ESR2
• Gene ID (NCBI): 2100
• RRID: AB_2881344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Pharmaceuticals (Basel); Isoliquiritigenin Inhibits the Growth of Colorectal Cancer Cells through the ESR2/PI3K/AKT Signalling Pathway; Authors - Fenglin Luo