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ESR2 Monoclonal antibody

ESR2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5D2C9

Cat no : 60197-1-Ig

Synonyms

ER BETA, Erb, ERβ, ESR BETA, ESR2, ESRB, ESTRB, estrogen receptor 2 (ER beta), Estrogen receptor beta, NR3A2



Tested Applications

Positive WB detected inMCF-7 cells, human brain tissue, SW 1990 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60197-1-Ig targets ESR2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ESR2 fusion protein Ag5103
Full Name estrogen receptor 2 (ER beta)
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC024181
Gene Symbol ESR2
Gene ID (NCBI) 2100
RRIDAB_10897162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Estrogen receptor-beta (ESR2) is a member of the superfamily of nuclear receptors, which can transduce extracellular signals into transcriptional responses. It binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. ESR2 exists various isoform and range of calculated molecular weight of isoforms is 50-60 kDa. The experiment detected two bands for ESR2 in isolated human germ cells, one at 60 kDa and a weaker one at 50 kDa (PMID: 14766008).

Protocols

Product Specific Protocols
WB protocol for ESR2 antibody 60197-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Environ Sci (China)

Environmental microcystin exposure triggers the poor prognosis of prostate cancer: Evidence from case-control, animal, and in vitro studies

Authors - Chun Pan
mouseWB

Environ Pollut

Betulinic acid alleviates zearalenone-induced uterine injury in mice

Authors - Chenglin Yang
humanWB

Biochim Biophys Acta Mol Cell Res

IL-17 promotes melanoma through TRAF2 as a scaffold protein recruiting PIAS2 and ELAVL1 to induce EPHA5

Authors - Junfeng Du
mouseWB,IHC,IF

Inflammation

High Dose of Estrogen Protects the Lungs from Ischemia-Reperfusion Injury by Downregulating the Angiotensin II Signaling Pathway

Authors - Peng Dai
mouseWB

Biofactors

Propofol exerts neuroprotective functions by down-regulating microRNA-19a in glutamic acid-induced PC12 cells.

Authors - Shashuang Yu
humanWB

PeerJ

The modulatory properties of Astragalus membranaceus treatment on endometrial cancer: an integrated pharmacological method.

Authors - Qianqian Zhang