ESM1 Polyclonal antibody

• 

  WB analysis of HUVEC using 12355-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 12355-1-AP (ESM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HUVEC using 12355-1-AP

  IP result of anti-ESM1 (IP:12355-1-AP, 4ug; Detection:12355-1-AP 1:300) with HUVEC cells lysate 640 ug.

ESM1 Polyclonal Antibody for WB, IP, ELISA

Cat No. 12355-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

ESM-1, Endothelial cell-specific molecule 1, Endothelial cell specific molecule 1, endothelial cell molecule 1, endocan

---

Tested Applications

• Positive WB detected in: HUVEC cells
• Positive IP detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12355-1-AP targets ESM1 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ESM1 fusion protein Ag3019
• Full Name: endothelial cell-specific molecule 1
• Calculated Molecular Weight: 184 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC011989
• Gene Symbol: ESM1
• Gene ID (NCBI): 11082
• RRID: AB_3669137
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQ30
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ESM1 is a secreted protein mainly expressed in the endothelial cells. The deduced 184-amino acid protein is cysteine rich with an N-terminal hydrophobic signal sequence. Expression of ESM1 was tested by Northern blot analysis and RT-PCR and was found to be constitutive in a HUVEC cell line, but not in any other cell line tested. Constitutive ESM1 expression in human tissue was restricted to lung.