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MIG6; ERRFI1 Polyclonal antibody

MIG6; ERRFI1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11630-1-AP

Synonyms

ERRFI1, Mitogen-inducible gene 6 protein, MIG-6, MIG6, MIG 6



Tested Applications

Positive WB detected inHCT 116 cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11630-1-AP targets MIG6; ERRFI1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MIG6; ERRFI1 fusion protein Ag2202
Full Name ERBB receptor feedback inhibitor 1
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC025337
Gene Symbol MIG6
Gene ID (NCBI) 54206
RRIDAB_2101525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERRFI1 (the product of mitogen-inducible gene 6, also known as MIG6) is an early response gene encoding a non-kinase scaffold adaptor protein induced by various stimuli such as hormones and stresses. ERRFI1 is considered a negative regulator of EGFR because it can directly bind to EGFR, inhibit the catalytic activity of EGFR, and mediate EGFR lysosomal degradation. ERRFI1 may play important roles in regulating stress response, maintaining homeostasis in tissues like joints or cardiac muscle, and functioning as a tumor suppressor.

Protocols

Product Specific Protocols
WB protocol for MIG6; ERRFI1 antibody 11630-1-APDownload protocol
IHC protocol for MIG6; ERRFI1 antibody 11630-1-APDownload protocol
IF protocol for MIG6; ERRFI1 antibody 11630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Biomed Eng

Profiling of protein-protein interactions via single-molecule techniques predicts the dependence of cancers on growth-factor receptors.

Authors - Hong-Won Lee
mouseIHC

Proc Natl Acad Sci U S A

Cartilage-specific deletion of Mig-6 results in osteoarthritis-like disorder with excessive articular chondrocyte proliferation.

Authors - Ben Staal
humanWB

Nucleic Acids Res

A genome landscape of SRSF3-regulated splicing events and gene expression in human osteosarcoma U2OS cells.

Authors - Masahiko Ajiro
humanWB

Aging Cell

METTL14 affects UVB-induced human dermal fibroblasts photoaging via miR-100-3p biogenesis in an m6 A-dependent manner

Authors - Lihao Chen
humanIHC

EMBO Rep

MIG-6 is essential for promoting glucose metabolic reprogramming and tumor growth in triple-negative breast cancer.

Authors - Jiabei He
humanWB

Arch Pharm Res

Selumetinib overcomes gefitinib primary and acquired resistance by regulating MIG6/STAT3 in NSCLC

Authors - Xiaoping Song
  • KD Validated