ERP29 Monoclonal antibody, PBS Only

ERP29 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1H8B2

Cat no : 67675-1-PBS

Synonyms

C12orf8, ERp28, ERP29, ERp31, PDI DB, PDIA9

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Validation Data Gallery

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  WB analysis using 67675-1-Ig (same clone as 67675-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67675-1-Ig (ERP29 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67675-1-Ig (ERP29 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ERP29 antibody (67675-1-Ig, Clone: 1H8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 67675-1-Ig (same clone as 67675-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using ERP29 antibody (67675-1-Ig, Clone: 1H8B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67675-1-PBS in a different storage buffer formulation.

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Product Information

67675-1-PBS targets ERP29 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERP29 fusion protein Ag30262
• Full Name: endoplasmic reticulum protein 29
• Calculated Molecular Weight: 261 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC101495
• Gene Symbol: ERP29
• Gene ID (NCBI): 10961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Endoplasmic reticulum resident protein 29 (ERP29) is a 29-kDa endoplasmic reticulum (ER) luminal protein. Structurally ERp29 is related to protein disulfide isomerases (PDI). However, it lacks the thioredoxin motif, suggesting that this protein does not function as a disulfide isomerase (PMID: 15500441). ERP29 plays an important role in the processing of secretory proteins within the (ER), possibly by participating in the folding of proteins in the ER. The secretion of thyroglobulin (TG) was found to be strongly regulated by ERP29 (PMID: 16380091).