ERN2 Monoclonal antibody, PBS Only

ERN2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G6A10

Cat no : 67289-1-PBS

Synonyms

ERN2, hIRE2p, Inositol requiring protein 2, Ire1 beta, IRE1b, IRE2

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Validation Data Gallery

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  WB analysis of JAR using 67289-1-Ig (same clone as 67289-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67289-1-Ig (ERN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67289-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67289-1-Ig (same clone as 67289-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67289-1-Ig (ERN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67289-1-Ig (same clone as 67289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67289-1-Ig (ERN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67289-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67289-1-Ig (same clone as 67289-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67289-1-Ig (ERN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67289-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67289-1-PBS targets ERN2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERN2 fusion protein Ag29452
• Full Name: endoplasmic reticulum to nucleus signaling 2
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC157113
• Gene Symbol: ERN2
• Gene ID (NCBI): 10595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.