ERMN Recombinant antibody

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  WB analysis using 84680-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84680-4-RR (ERMN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ERMN Recombinant Antibody for WB, ELISA

Cat No. 84680-4-RR

Clone No.242164C3

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

KIAA1189, Juxtanodin, JN, Ermin, 242164C3

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Tested Applications

• Positive WB detected in: rat brain tissue, rat cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84680-4-RR targets ERMN in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: ERMN fusion protein Ag35648
• Full Name: ermin, ERM-like protein
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC026345
• Gene Symbol: ERMN
• Gene ID (NCBI): 57471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q8TAM6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERMN is associated with cytoskeletal rearrangements and the stability of myelin sheath. A study has shown that ERMN is expressed specifically in oligodendrocytes in the central nervous system (CNS), and is involved in the formation of myelin (PMID: 20934411). The predicted MW of ERMN is 33 KDa, while western blot analyses detected a 50 kDa single band protein as a result of phosphorylation modification.