ERMN Monoclonal antibody

ERMN Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2B1C1

Cat no : 66605-1-Ig

Synonyms

ermin, ermin, ERM like protein, ERMN, JN, Juxtanodin, KIAA1189

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Validation Data Gallery

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  WB analysis using 66605-1-Ig

  Various tissues were subjected to SDS PAGE followed by western blot with 66605-1-Ig (ERMN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66605-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66605-1-Ig (ERMN antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: pig brain tissue, pig cerebellum, rat brain, rat cerebellum, mouse brain
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

66605-1-Ig targets ERMN in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERMN fusion protein Ag27040
• Full Name: ermin, ERM-like protein
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC026345
• Gene Symbol: ERMN
• Gene ID (NCBI): 57471
• RRID: AB_2881965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERMN is associated with cytoskeletal rearrangements and the stability of myelin sheath. A study has shown that ERMN is expressed specifically in oligodendrocytes in the central nervous system (CNS), and is involved in the formation of myelin (PMID: 20934411). The predicted MW of ERMN is 33 KDa, while western blot analyses of different tissue lysates using 66605-1-Ig antibody to ERMN detected a 50 kDa single band protein as a result of phosphorylation modification.

Publications

Species	Application	Title
human	IHC	Bioengineered; Four specific biomarkers associated with the progression of glioblastoma multiforme in older adults identified using weighted gene co-expression network analysis; Authors - Yushi Yang
mouse	WB	Prog Neurobiol; Altered activity-regulated H3K9 acetylation at TGF-beta signaling genes during egocentric memory in Huntington's disease; Authors - Rafael Alcalá-Vida