ERMN Polyclonal antibody

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  WB analysis using 31432-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31432-1-AP (ERMN antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 31432-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using ERMN antibody (31432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 31432-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using ERMN antibody (31432-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ERMN Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 31432-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF-P, ELISA

Synonyms

KIAA1189, Juxtanodin, JN, Ermin

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Tested Applications

• Positive WB detected in: rat brain tissue, rat cerebellum tissue
• Positive IF-P detected in: rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31432-1-AP targets ERMN in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERMN fusion protein Ag35648
• Full Name: ermin, ERM-like protein
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC026345
• Gene Symbol: ERMN
• Gene ID (NCBI): 57471
• RRID: AB_3669981
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8TAM6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERMN is associated with cytoskeletal rearrangements and the stability of myelin sheath. A study has shown that ERMN is expressed specifically in oligodendrocytes in the central nervous system (CNS), and is involved in the formation of myelin (PMID: 20934411). The predicted MW of ERMN is 33 KDa, while western blot analyses detected a 45-50 kDa single band protein as a result of phosphorylation modification.