ERLIN1 Polyclonal antibody

ERLIN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 17311-1-AP

Synonyms

KE04, Erlin-1, Erlin 1, ER lipid raft associated 1, Endoplasmic reticulum lipid raft-associated protein 1

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Validation Data Gallery

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  WB analysis using 17311-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 17311-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human ovary cancer using 17311-1-AP

  human ovary cancer were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat pancreas using 17311-1-AP

  rat pancreas tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 17311-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17311-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 17311-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17311-1-AP (ERLIN1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 17311-1-AP

  IP result of anti-ERLIN1 (IP:17311-1-AP, 3ug; Detection:17311-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse liver using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17311-1-AP (ERLIN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17311-1-AP (ERLIN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17311-1-AP (ERLIN1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17311-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17311-1-AP (ERLIN1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HeLa using 17311-1-AP

  Immunofluorescent analysis of HeLa cells using 17311-1-AP (ERLIN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HL-60 cells, COLO 320 cells, human kidney tissue, human ovary cancer cells, rat pancreas tissue, human brain tissue, mouse liver tissue, U-251 cells, U-397 cells
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: mouse liver tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

17311-1-AP targets ERLIN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERLIN1 fusion protein Ag11279
• Full Name: ER lipid raft associated 1
• Calculated Molecular Weight: 346 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC031791
• Gene Symbol: ERLIN1
• Gene ID (NCBI): 10613
• RRID: AB_2098590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell; BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.; Authors - Fabien Llambi
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex; Authors - Michael L van de Weijer
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.; Authors - Michael L van de Weijer
human	WB	Elife; RNF41 regulates the damage recognition receptor Clec9A and antigen cross-presentation in mouse dendritic cells.; Authors - Kirsteen M Tullett
human	WB	PLoS Genet; Disruption of the ERLIN-TM6SF2-APOB complex destabilizes APOB and contributes to non-alcoholic fatty liver disease.; Authors - Bo-Tao Li
human	IHC,WB	Front Mol Biosci; Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients; Authors - Liping Shi