ERK1/2 Monoclonal antibody

ERK1/2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (4)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

67

CloneNo.

1A3D3

Reviews (1)

Cat no : 67170-1-Ig

Synonyms

ERK, ERK 1, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

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Validation Data Gallery

• 

  WB analysis of PC-12 using 67170-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of ROS1728 using 67170-1-Ig

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 67170-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 67170-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67170-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67170-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 67170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67170-1-Ig (ERK1/2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 67170-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67170-1-Ig (ERK1/2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: PC-12 cells, HeLa cells, Jurkat cells, RAW 264.7 cells, ROS1728 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 65 publications below
• IHC: See 1 publications below

Product Information

67170-1-Ig targets ERK1/2 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: mitogen-activated protein kinase 3
• Calculated Molecular Weight: 38-43 kDa
• GenBank Accession Number: NM_002746
• Gene Symbol: ERK1
• Gene ID (NCBI): 5595
• RRID: AB_2882466
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.

Publications

Species	Application	Title
		Mol Cell; PARP5A and RNF146 phase separation restrains RIPK1-dependent necroptosis; Authors - Shouqiao Hou
rat	WB	J Hazard Mater; ERK/p38/ROS burst responses to environmentally relevant concentrations of diphenyl phosphate-evoked neutrophil extracellular traps formation: Assessing the role of autophagy.; Authors - Siwen Li
mouse	WB	J Hazard Mater; Prenatal exposure to environmentally relevant levels of PBDE-99 leads to testicular dysgenesis with steroidogenesis disorders.; Authors - Tianxin Zhao
mouse	WB	Cancer Lett; SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.; Authors - Tian-Jiao Li
human	WB	Int J Mol Sci; LMNA Reduced Acquired Resistance to Erlotinib in NSCLC by Reversing the Epithelial–Mesenchymal Transition via the FGFR/MAPK/c-fos Signaling Pathway; Authors - Chunsheng Hu
mouse	WB	J Neuroinflammation; Regulatory coupling between long noncoding RNAs and senescence in irradiated microglia.; Authors - Anan Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (05-18-2020)

Excellent Antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: AC16

ERK1/2 Antibody Western Blot validation (1:1000 dilution) in AC16 (Cat no:67170-1-Ig)