ERK1/2 Monoclonal antibody, PBS Only

ERK1/2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4G8A11

Cat no : 66192-1-PBS

Synonyms

ERK, ERK 1, ERK 2, ERK1, ERK1/2, ERK2, ERT2, HS44KDAP, HUMKER1A, MAP kinase 1, MAP kinase 3, MAP kinase isoform p44, MAPK 1, MAPK 3, MAPK3, p44 ERK1, p44 MAPK, P44ERK1, P44MAPK, PRKM3

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Validation Data Gallery

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  WB analysis using 66192-1-Ig (same clone as 66192-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66192-1-Ig (same clone as 66192-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66192-1-Ig (same clone as 66192-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66192-1-Ig (same clone as 66192-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66192-1-Ig (ERK1/2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0. This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66192-1-Ig (same clone as 66192-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ERK1/2 antibody (66192-1-Ig, Clone: 4G8A11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66192-1-PBS in a different storage buffer formulation.

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Product Information

66192-1-PBS targets ERK1/2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERK1/2 fusion protein Ag20350
• Full Name: mitogen-activated protein kinase 3
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 38-44 kDa
• GenBank Accession Number: BC013992
• Gene Symbol: ERK1
• Gene ID (NCBI): 5595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.