ERI1 Polyclonal antibody

ERI1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14592-1-AP

Synonyms

HEXO, Eri-1 homolog, ERI 1, EC:3.1.-.-, 3'EXO

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Validation Data Gallery

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  WB analysis of K-562 using 14592-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14592-1-AP (ERI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 14592-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14592-1-AP (ERI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14592-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14592-1-AP (ERI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14592-1-AP (ERI1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 14592-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14592-1-AP (ERI1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 14592-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14592-1-AP (ERI1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 14592-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14592-1-AP (ERI1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 14592-1-AP

  Immunofluorescent analysis of HepG2 cells using 14592-1-AP (ERI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: K-562 cells, HepG2 cells, HL-60 cells
• Positive IHC detected in: human liver cancer tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14592-1-AP targets ERI1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERI1 fusion protein Ag6125
• Full Name: exoribonuclease 1
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: BC035279
• Gene Symbol: ERI1
• Gene ID (NCBI): 90459
• RRID: AB_11182393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERI1, also named as HEXO, 3'HEXO and THEX1, contains one exonuclease domain and one SAP domain. The SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity. It is a RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. It is able to bind other mRNAs. ERI1 is required for 5.8S rRNA 3'-end processing. It also binds to 5.8s ribosomal RNA. ERI1 binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.