ERGIC3 Monoclonal antibody

• 

  WB analysis using 68398-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68398-1-Ig (ERGIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 68398-1-Ig

  WB result of ERGIC3 antibody (68398-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERGIC3 transfected HepG2 cells.

ERGIC3 Monoclonal Antibody for WB, ELISA

Cat No. 68398-1-Ig

Clone No.1B9F11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ERV46, Endoplasmic reticulum-Golgi intermediate compartment protein 3, CGI-54, CGI 54, C20orf47

---

Tested Applications

• Positive WB detected in: NCI-H1299 cells, HepG2 cells, Calu-3 cells, U2OS cells, LNCaP cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68398-1-Ig targets ERGIC3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERGIC3 fusion protein Ag9190
• Full Name: ERGIC and golgi 3
• Calculated Molecular Weight: 383 aa, 43 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC009765
• Gene Symbol: ERGIC3
• Gene ID (NCBI): 51614
• RRID: AB_3085115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9Y282
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERGIC3 (Endoplasmic reticulum-Golgi intermediate compartment protein 3 ) is located in the cis face of the Golgi apparatus and vesicular tubular structures between the transitional endoplasmic reticulum (ER) and cis-Golgi. ERGIC3 significantly affects cell growth and causes ER stress-induced cell death, and is involved in the invasion and metastasis in hepatocellular carcinomas (HCC). (PMID: 26177443, PMID: 31142615)