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ERG Polyclonal antibody
ERG Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 14356-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, HEK-293 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human prostate cancer tissue, mouse brain tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
14356-1-AP targets ERG in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ERG fusion protein Ag5708 |
Full Name | v-ets erythroblastosis virus E26 oncogene homolog (avian) |
Calculated Molecular Weight | 55 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC040168 |
Gene Symbol | ERG |
Gene ID (NCBI) | 2078 |
RRID | AB_2098423 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ERG (ETS-related gene) is a member of the ETS family of transcription factors, which are downstream effectors of mitogenic signaling transduction pathway. ERG is a key regulator of cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. ERG is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ERG antibody 14356-1-AP | Download protocol |
IHC protocol for ERG antibody 14356-1-AP | Download protocol |
IF protocol for ERG antibody 14356-1-AP | Download protocol |
IP protocol for ERG antibody 14356-1-AP | Download protocol |
FC protocol for ERG antibody 14356-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Biosci Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer | ||
J Cancer miR-223-5p targeting ERG inhibits prostate cancer cell proliferation and migration.
| ||
Cell Death Discov HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells. | ||
Exp Ther Med Rare angiolymphoid hyperplasia with eosinophilia examined through fine needle aspiration cytology, histopathology and immunophenotypic characterization: A case report | ||
Nat Commun DNA framework-engineered chimeras platform enables selectively targeted protein degradation |