ERG Polyclonal antibody

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  WB analysis of MCF-7 using 14356-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 14356-1-AP (ERG Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 14356-1-AP

  IP result of anti-ERG (IP:14356-1-AP, 4ug; Detection:14356-1-AP 1:500) with MCF-7 cells lysate 800ug.

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  IP experiment of rat heart using 14356-1-AP

  IP result of anti-ERG (IP:14356-1-AP, 4ug; Detection:14356-1-AP 1:500) with rat heart tissue lysate 2240 ug.

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  IHC staining of human prostate cancer using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 14356-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14356-1-AP (ERG antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 14356-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using ERG antibody (14356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of MG-63 using 14356-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MG-63 cells using ERG antibody (14356-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

ERG Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14356-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

erg 3, Transcriptional regulator ERG, Transforming protein ERG

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Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IP detected in: MCF-7 cells, rat heart tissue
• Positive IHC detected in: human prostate cancer tissue, mouse brain tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MG-63 cells, HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:600-1:2400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

14356-1-AP targets ERG in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5708

  Product name: Recombinant human ERG protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 141-479 aa of BC040168

  Sequence: QWLEWAVKEYGLPDVNILLFQNIDGKELCKMTKDDFQRLTPSYNADILLSHLHYLRETPLPHLTSDDVDKALQNSPRLMHARNTGGAAFIFPNTSVYPEATQRITTRPDLPYEPPRRSAWTGHGHPTPQSKAAQPSPSTVPKTEDQRPQLDPYQILGPTSSRLANPGSGQIQLWQFLLELLSDSSNSSCITWEGTNGEFKMTDPDEVARRWGERKSKPNMNYDKLSRALRYYYDKNIMTKVHGKRYAYKFDFHGIAQALQPHPPESSLYKYPSDLPYMGSYHAHPQKMNFVAPHPPALPVTSSSFFAAPNPYWNSPTGGIYPNTRLPTSHMPSHLGTYY

• Full Name: v-ets erythroblastosis virus E26 oncogene homolog (avian)
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC040168
• Gene Symbol: ERG
• Gene ID (NCBI): 2078
• RRID: AB_2098423
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11308
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERG (ETS-related gene) is a member of the ETS family of transcription factors, which are downstream effectors of mitogenic signaling transduction pathway. ERG is a key regulator of cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. ERG is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Stem Cell; PSPC1 exerts an oncogenic role in AML by regulating a leukemic transcription program in cooperation with PU.1; Authors - Juyeong Hong
mouse	IF	iScience; Resident vascular Sca1+ progenitors differentiate into endothelial cells in vascular remodeling via miR-145-5p/ERG signaling pathway; Authors - Zhangquan Ying
human	WB	Cell Biosci; Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer; Authors - Qiong Wang
human	WB	J Cancer; miR-223-5p targeting ERG inhibits prostate cancer cell proliferation and migration.; Authors - Yongbao Wei ; KD Validated
mouse,human	WB,IF	Cell Death Discov; HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells.; Authors - Feng Yao
human	IHC	Exp Ther Med; Rare angiolymphoid hyperplasia with eosinophilia examined through fine needle aspiration cytology, histopathology and immunophenotypic characterization: A case report; Authors - Xiaohua Wang