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  • KD/KO Validated

ERG Polyclonal antibody

ERG Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 14356-1-AP


ERG, erg 3, p55, Transcriptional regulator ERG, Transforming protein ERG

Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue, mouse brain tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:600-1:2400
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14356-1-AP targets ERG in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ERG fusion protein Ag5708
Full Name v-ets erythroblastosis virus E26 oncogene homolog (avian)
Calculated Molecular Weight 55 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC040168
Gene Symbol ERG
Gene ID (NCBI) 2078
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERG (ETS-related gene) is a member of the ETS family of transcription factors, which are downstream effectors of mitogenic signaling transduction pathway. ERG is a key regulator of cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. ERG is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia.


Product Specific Protocols
WB protocol for ERG antibody 14356-1-APDownload protocol
IHC protocol for ERG antibody 14356-1-APDownload protocol
IF protocol for ERG antibody 14356-1-APDownload protocol
IP protocol for ERG antibody 14356-1-APDownload protocol
FC protocol for ERG antibody 14356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Biosci

Profile of chimeric RNAs and TMPRSS2-ERG e2e4 isoform in neuroendocrine prostate cancer

Authors - Qiong Wang

J Cancer

miR-223-5p targeting ERG inhibits prostate cancer cell proliferation and migration.

Authors - Yongbao Wei
  • KD Validated

Cell Death Discov

HDAC11 promotes both NLRP3/caspase-1/GSDMD and caspase-3/GSDME pathways causing pyroptosis via ERG in vascular endothelial cells.

Authors - Feng Yao

Exp Ther Med

Rare angiolymphoid hyperplasia with eosinophilia examined through fine needle aspiration cytology, histopathology and immunophenotypic characterization: A case report

Authors - Xiaohua Wang

Nat Commun

DNA framework-engineered chimeras platform enables selectively targeted protein degradation

Authors - Li Zhou