ERCC6L Polyclonal antibody

• Featured Product
• KD/KO Validated

ERCC6L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15688-1-AP

Synonyms

Tumor antigen BJ HCC 15, PLK1-interacting checkpoint helicase, PICH, EC:3.6.4.12, DNA excision repair protein ERCC-6-like

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Validation Data Gallery

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  WB analysis using 15688-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15688-1-AP (ERCC6L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 15688-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15688-1-AP (ERCC6L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15688-1-AP

  WB result of ERCC6L antibody (15688-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ERCC6L transfected Jurkat cells.

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  WB analysis of mouse lung using 15688-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15688-1-AP (ERCC6L antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15688-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15688-1-AP (ERCC6L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 15688-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15688-1-AP (ERCC6L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human heart tissue, Jurkat cells, mouse lung tissue, K-562 cells, U2OS cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Product Information

15688-1-AP targets ERCC6L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERCC6L fusion protein Ag8223
• Full Name: excision repair cross-complementing rodent repair deficiency, complementation group 6-like
• Calculated Molecular Weight: 419 aa, 46 kDa, 141 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC008808
• Gene Symbol: ERCC6L
• Gene ID (NCBI): 54821
• RRID: AB_2098171
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERCC6L, also named as Tumor antigen BJ-HCC-15 or PICH, is a 1250 amino acid protein, which contains one helicase ATP-binding domain, one helicase C-terminal domain and two TPR repeats. ERCC6L localizes to kinetochores, inner centromeres and belongs to the SNF2/RAD54 helicase family. ERCC6L as a DNA helicase that acts as an essential component of the spindle assembly checkpoint. The calculated molecular weight of ERCC6L is 140 kDa, but the phosphorylated modification of molecular weight of ERCC6L is about 180kDa.

Publications

Species	Application	Title
human	WB	Eur J Med Res; Potential 'anti-cancer' effects of esketamine on proliferation, apoptosis, migration and invasion in esophageal squamous carcinoma cells; Authors - Chao Li
human,mouse	WB	J Ethnopharmacol; Gypenoside LI arrests the cell cycle of breast cancer in G0/G1 phase by down-regulating E2F1.; Authors - Ma-Li Zu
human	WB	Oncol Rep; Overexpression of ERCC6L correlates with poor prognosis and confers malignant phenotypes of lung adenocarcinoma.; Authors - Xiaoyue Huang
human	WB	BMC Cancer; ERCC6L promotes the progression of hepatocellular carcinoma through activating PI3K/AKT and NF-κB signaling pathway.; Authors - Han Chen ; KD Validated
human	WB,IHC	Oncol Lett; ERCC6L promotes cell growth and invasion in human colorectal cancer.; Authors - Yang Xie ; KD Validated
human	WB,IHC	Cancer Res; PICH activates Cyclin A1 transcription to drive S-phase progression and chemoresistance in gastric cancer; Authors - Huili Ye