ERC1 Monoclonal antibody, PBS Only

ERC1 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A5B9

Cat no : 66681-1-PBS

Synonyms

Cast2, ELKS, ERC 1, ERC1, KIAA1081, Rab6 interacting protein 2, RAB6IP2

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Validation Data Gallery

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  WB analysis using 66681-1-Ig (same clone as 66681-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66681-1-Ig (ERC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66681-1-PBS in a different storage buffer formulation.

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Product Information

66681-1-PBS targets ERC1 in WB, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ERC1 fusion protein Ag17665
• Full Name: ELKS/RAB6-interacting/CAST family member 1
• Calculated Molecular Weight: 1116 aa, 128 kDa
• Observed Molecular Weight: 135 kDa
• GenBank Accession Number: BC132782
• Gene Symbol: ERC1
• Gene ID (NCBI): 23085
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ERC is an acronym based on previous separate namings of members of this protein family, ELKS, Rab6IP2 and CAST (PMID: 12391317). They are known to bind RIMs, the active zone proteins that regulate neurotransmitter release. ERC1 (ELKS) is an essential regulatory subunit of the IKK complex and likely functions by recruiting IκBα to the complex (PMID: 15218148). Multiple isoforms of ERC1 mRNA are generated by alternative splicing (PMID: 12203787). There are two known splice variants of ERC1 protein that differ at their C termini, ERC1a and ERC1b. ERC1a is ubiquitously expressed, whereas ERC1b is brain-specific (PMID: 12391317; 12923177).