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HER2/ErbB2,p185-Specific Recombinant antibody, PBS Only

HER2/ErbB2,p185-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240802C2

Cat no : 84046-1-PBS

Synonyms

ERBB2, HER2, HER 2/neu, HER 2, CD340

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HER2/ErbB2,p185-Specific Recombinant antibody (84046-1-RR)

    WB analysis using 84046-1-RR (same clone as 84046-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84046-1-RR (ERBB2,p185-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovarian cancer using HER2/ErbB2,p185-Specific Recombinant antibody (84046-1-RR)

    IHC staining of human ovarian cancer using 84046-1-RR (same clone as 84046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84046-1-RR (HER2/ErbB2,p185-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse uterus tissue using HER2/ErbB2,p185-Specific Recombinant antibody (84046-1-RR)

    IHC staining of mouse uterus using 84046-1-RR (same clone as 84046-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse uterus tissue slide using 84046-1-RR (HER2/ErbB2,p185-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84046-1-PBS in a different storage buffer formulation.

Product Information

84046-1-PBS targets HER2/ErbB2,p185-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
Calculated Molecular Weight 138 kDa
Observed Molecular Weight185 kDa
GenBank Accession NumberBC167147
Gene Symbol HER2/ErbB2
Gene ID (NCBI) 2064
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.