ERAP2 Polyclonal antibody

ERAP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14637-1-AP

Synonyms

ERAP2, L RAP, LRAP

---

Validation Data Gallery

• 

  WB analysis of K-562 using 14637-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14637-1-AP (ERAP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14637-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14637-1-AP (ERAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 14637-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14637-1-AP (ERAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 14637-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 14637-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 14637-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 14637-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14637-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 14637-1-AP (ERAP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: K-562 cells, A549 cells, mouse thymus tissue
• Positive IHC detected in: human lung cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14637-1-AP targets ERAP2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERAP2 fusion protein Ag6216
• Full Name: endoplasmic reticulum aminopeptidase 2
• Calculated Molecular Weight: 110 kDa
• Observed Molecular Weight: 61-65 kDa
• GenBank Accession Number: BC065240
• Gene Symbol: ERAP2
• Gene ID (NCBI): 64167
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ERAP2(Endoplasmic reticulum aminopeptidase 2) is also named as LRAP(Leukocyte-derived arginine aminopeptidase) and belongs to the peptidase M1 family. It plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. It can form heterodimers with ERAP1(PMID:15908954). It has 4 isoforms produced by alternative splicing. And defects in the expression of this gene may cause improper antigen processing, possibly leading to favor tumor escape from the immune surveillance.