ERAL1 Polyclonal antibody, PBS Only

ERAL1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29465-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HEK-293T using 29465-1-AP (same clone as 29465-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 29465-1-AP (ERAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29465-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 29465-1-AP (same clone as 29465-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 29465-1-AP (ERAL1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29465-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 29465-1-AP (same clone as 29465-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29465-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 29465-1-AP (same clone as 29465-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29465-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 29465-1-AP (same clone as 29465-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29465-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 29465-1-AP (same clone as 29465-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29465-1-AP (ERAL1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29465-1-PBS in a different storage buffer formulation.

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Product Information

29465-1-PBS targets ERAL1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ERAL1 fusion protein Ag30720
• Full Name: Era G-protein-like 1 (E. coli)
• Calculated Molecular Weight: 437 aa, 48 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC019094
• Gene Symbol: ERAL1
• Gene ID (NCBI): 26284
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

ERAL1, a homologue of Era protein in Escherichia coli, is a member of conserved GTP-binding proteins with RNA-binding activity. ERAL1 localizes to the mitochondrionmitoribosomal subunit, and binds to the 3' terminal stem loop of 12S mitochondrial rRNA and is required for proper assembly of the 28S small mitochondrial ribosomal subunit. Deletion of ERAL1 has been shown to cause mitochondrial dysfunction, growth retardation, and apoptosis.