AHSP Polyclonal antibody

AHSP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13643-1-AP

Synonyms

ERAF, Erythroid-associated factor, Erythroid differentiation-related factor, erythroid associated factor, EDRF

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Validation Data Gallery

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  WB analysis using 13643-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13643-1-AP (AHSP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 13643-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13643-1-AP (AHSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 13643-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13643-1-AP (AHSP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: pig bone marrow tissue
• Positive IHC detected in: human placenta tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13643-1-AP targets AHSP in WB, IHC, ELISA applications and shows reactivity with human, mouse, pig samples.

• Tested Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AHSP fusion protein Ag4588
• Full Name: erythroid associated factor
• Calculated Molecular Weight: 102 aa, 12 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC035842
• Gene Symbol: AHSP
• Gene ID (NCBI): 51327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AHSP acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development, and specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia (PMID: 12066189).